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ASK® ALKALINE PHOSPHATASE (Alk-P) LIQUID REAGENT
PURPOSE
The ASK ALKALINE PHOSPHATASE (Alk-P) LIQUID REAGENT is, a kinetic method, used for the quantitative determination of alkaline phosphatase (Alk-P) in serum.
PRINCIPLE
Alk. Phos.
p-Npp + H2O ---------------> p-Nitrophenol + H3PO4
p-Npp is colorless but p-Nitrophenol has strong absorbance at 405 nm. The rate of increased absorbance at 405nm is proportional to the enzyme activity.