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ASK® HDL-CHOLESTEROL LIQUID REAGENT (Non-Sterile)
PURPOSE
The ASK HDL-CHO LIQUID REAGENT (direct method) is, Enzymatic end point method, used for the quantitative determination of high density lipoprotein - Cholesterol in serum.
PRINCIPLE
Detergent
LDL, VLDL, CM ------------------- > Complexes
Polyanion
Detergent
HDL --------------------------- > HDL (disrupted)
Cholesterol esterase
HDL cholesterol ---------------------------------------- > Cholestenon + H2O2
Cholesterol oxidase
peroxidase
H2O2 + 4-aminoantipyrine +DSBmT ------------------- > Reddish purple compound
The amount of complex formed is proportional to HDL-CHO concentration and can be photometrically measured.