Product List > Medical device > Clinical chemistry reage > ASK® TRIGLYCERIDES GPO LIQUID REAGENT (Non-Sterile)
ASK® TRIGLYCERIDES GPO LIQUID REAGENT (Non-Sterile)
PURPOSE
The ASK TRIGLYCERIDES GPO LIQUID REAGENT is, an end point method, used for the quantitative determination of triglycerides in serum.
PRINCIPLE
Lipase
Triglycerides --------------> Glycerol + Fatty Acids
Glycerol Kinase
Glycerol + ATP ----------------------> Glycerol-1-phosphate + ADP
GPO
Glycerol-1-phosphate + O2 -------------------> DAP + H2O2
Peroxidase
H2O2 + 4-AA + DHBS -------------------> Quinoneimine Dye + H2O
The present procedure involves hydrolysis of triglycerides by lipase. The glycerol concentration is then determined by enzymatic assay coupled with Trinder reaction that terminates in the formation of a quinoneimine dye. The amount of the dye formed, determined by its absorption at 520nm, is directly proportional to the concentration of triglycerides in the samples.