Product List > Medical device > Clinical chemistry reage > ASK® CHOLESTEROL LIQUID REAGENT (Non-Sterile)
ASK® CHOLESTEROL LIQUID REAGENT (Non-Sterile)
PURPOSE
The ASK CHOLESTEROL LIQUID REAGENT is, an end point method, used for the quantitative determination of total cholesterol in serum.
PRINCIPLE
C. Esterase
Cholesterol Esters --------------> Cholesterol + Fatty acids
C. oxidase
Cholesterol + O2 --------------> Cholesten-3-one + H2O2
H. peroxidase
2H2O2 + 4-Aminoantipyrine + p-HBS ------------------> Quinoneimine + 2H2O
Cholesterol esters are hydrolyzed to produce cholesterol. Hydrogen peroxide is then produced from the oxidation of cholesterol by cholesterol oxidase. In a coupled reaction catalyzed by peroxidase, quinoneimine dye colored red is formed from 4-aminoantipyrine, p-HBS and hydrogen peroxide. The absorption at 520nm of the solution of this dye is proportional to the concentration of cholesterol in the sample.